Effect Of Plant Extracts On H2 O2 -induced Infl...

Creatine Supplementation Upregulates Excitation...

0 votes
Creatine Supplementation Upregulates Excitation-contraction Coupling In C2c12 Myotubes
Author: Aida Calderón-rivera, Ana Victoria Vega, Guillermo Avila
Publisher: Derivative Works
9 pages
One time payment: €0.00
Required subscription: Free
Type of publication: Article
ISBN/ISSN: 1178-699X
DOI: 10.2147/JRLCR.S6595
Follow this publisher

Share this publication:


Abstract: The main goal of this work was to investigate whether creatine (Cr) might be able to regulate skeletal muscle excitation-contraction (EC) coupling. Myotubes from a C2C12 cell line were exposed to Cr (25 mM, 2–4 hours). Subsequently, the activity of L-type Ca2+ channels and voltage-gated Ca2+ release (VGCR) were investigated using the whole-cell patch-clamp technique. Cr upregulated VGCR by 2.4-fold, in the absence of major alterations in L-type Ca2+ channel activity, the extent of caffeine-induced sarcoplasmic reticulum Ca2+ release, or the termination kinetics of Ca2+ transients. Thus, stimulation of VGCR cannot be explained by upregulation of the activity of either L-type Ca2+ channels or sarcoplasmic reticulum Ca2+-ATPase. We also investigated possible long-term regulation of L-type Ca2+ channels by Cr. However, chronic treatment with Cr (2–4 days) affected neither the density of ICaL nor the corresponding voltagedependence of activation. The later result was obtained in the face of a 1.7-fold stimulation of myogenesis. Therefore, it cannot be explained by possible desensitization of Cr metabolism. These data could suggest that the functional expression of L-type Ca2+ channels is not affected by prior acute stimulation of VGCR. Previous work has shown that Cr supplementation enhances muscle strength. Thus, it will be interesting to investigate whether this effect can be at least partially explained by acute stimulation of VGCR.

About the publisher:

We are a publishing house devoted to reuse CC-BY licensed published materials.


Using CC-BY licenses:


  • Adapt — remix, transform, and build upon the material
  • for any purpose, even commercially.
  • The licensor cannot revoke these freedoms as long as you follow the license terms.


  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others
    from doing anything the license permits.


  • You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
  • No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.

Select a payment method