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Detection Of Genes Tem, Oxa, Shv And Ctx-m In 73 Clinical Isolates Of Escherichia Coli Producers Of Extended Spectrum Beta- Lactamases And Determination Of Their Susceptibility To Antibiotics.
Author: Tabbouche Sana, Khudary Rami, Beyrouthy Racha1,6, Dabboussi Fouad, Achkar Marcel, Mallat Hassan, Hlais Sani,hamze Monzer
Publisher: Internet Medical Publishing
6 pages
One time payment: €0.00
Required subscription: Free
Type of publication: Article
DOI: 10:3823/704
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A total of 73 clinical isolates of extended spectrum β- lactamase producing  Escherichia coli were sampled in North Lebanon. The ESBL resistance was screened using disc diffusion method, while the resistance genes were detected by poly- merase chain reaction (PCR).

The results marked the high prevalence of gene CTX-M (72 strains), whereas 33 strains carried the gene OXA, 16 strains carried the gene TEM and 3 carried the SHV gene. The majority of the strains carried two or more genes. This study points out the high rate of transmission of these genes among Escherichia coli strains in North Lebanon and shows a reduced sensitivity to quinolones (pipemidic acid(12.1%); ofloxacin (21.9%) and ciprofloxacin (23.9%), in addition, all tested strains were highly susceptible to tigecycline (100%), fosfomycin (98.6%) and imipenem (97.2%)

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