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A total of 12 Acinetobacter baumannii isolates have been recovered from hospitalized patients at two hospitals in Jordan over two different periods of time (2006 and 2008). Phenotypic and biochemical characterization with antibiotic susceptibility testing indicated that alln isolates were belonging to A. baumannii. A high degree of conservation of both the 16S-23S rRNA gene intergenic spacer (ITS) length and the ITS sequence was observed among the isolates, and their identities were further confimed by amplifid ribosomal DNA gene restriction analysis (ARDRA). The application of ITS sequence-based identification and ARDRA provide promising tools for the elucidation of the clinical signifiance of genospecies identifiation of A. baumannii.
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